Afweersysteem 2011

Up-regulation of TGF-β1 mRNA expression in peripheral blood mononuclear cells of patients with chronic fatigue syndrome.

J Formos Med Assoc. 2011 Nov;110(11):701-4. doi:10.1016/j.jfma.2011.09.006.

Zhang HY, Liu ZD, Hu CJ, Wang DX, Zhang YB, Li YZ.

BACKGROUND/PURPOSE:

It has been shown that the abnormality

in immune cells in chronic fatigue syndrome (CFS) patients

is closely associated with the participation of TGF-β.

In order to study the relationship between TGF-β1 and CFS,

we investigated the mRNA levels of TGF-β1

in peripheral blood mononuclear cells (PBMCs) in patients with CFS.

METHODS:

Fluorescent quantitative real time reverse-transcription

polymerase chain reaction (FQ-RT-PCR) was performed to test

TGF-β1 mRNA expression in PBMCs

in 63 cases of CFS, 50 cases of disease controls, and 50 cases of healthy controls.

RESULTS:

The mean value of TGF-β1 mRNA expression in CFS patients was ΔΔCt=1.17±0.58,

which was significantly higher than

the disease controls (ΔΔCt=0.07±1.08, df=111, p < 0.01) and

the healthy controls (ΔΔCt=0.00±1.63, df=111, p < 0.01).

No significant difference was detected

between disease and healthy controls (p > 0.05).

CONCLUSION:

The expression of TGF-β1 in PBMCs

is significantly elevated in patients with CFS.

It might be correlated to the pathogenesis of the disease.

PMID: 22118314