Up-regulation of TGF-β1 mRNA expression in peripheral blood mononuclear cells of patients with chronic fatigue syndrome.
J Formos Med Assoc. 2011 Nov;110(11):701-4. doi:10.1016/j.jfma.2011.09.006.
Zhang HY, Liu ZD, Hu CJ, Wang DX, Zhang YB, Li YZ.
It has been shown that the abnormality
in immune cells in chronic fatigue syndrome (CFS) patients
is closely associated with the participation of TGF-β.
In order to study the relationship between TGF-β1 and CFS,
we investigated the mRNA levels of TGF-β1
in peripheral blood mononuclear cells (PBMCs) in patients with CFS.
Fluorescent quantitative real time reverse-transcription
polymerase chain reaction (FQ-RT-PCR) was performed to test
TGF-β1 mRNA expression in PBMCs
in 63 cases of CFS, 50 cases of disease controls, and 50 cases of healthy controls.
The mean value of TGF-β1 mRNA expression in CFS patients was ΔΔCt=1.17±0.58,
which was significantly higher than
the disease controls (ΔΔCt=0.07±1.08, df=111, p < 0.01) and
the healthy controls (ΔΔCt=0.00±1.63, df=111, p < 0.01).
No significant difference was detected
between disease and healthy controls (p > 0.05).
The expression of TGF-β1 in PBMCs
is significantly elevated in patients with CFS.
It might be correlated to the pathogenesis of the disease.