Brenu en collega's vergeleken de concentraties microRNAs (miRNAs)

in het plasma van 20 CVS-patiënten en 20 gezonde proefkonijnen.

miRNAs zijn stukjes DNA die niet tot een proteïne leiden, maar wel de genexpressie beïnvloeden.

De hoeveelheden miR-127-3p, miR-142-5p en miR-143-3p waren beduidend hoger in CVS.

miR-142-5p wordt in verband gebracht met immuunsysteem-ziekten en inflammatie,

miR-143-3p is mogelijk gerelateerd aan apoptose ("zelfmoord") van neutrofielen en andere cellen, en.

miR-127-3p wordt niet alleen gerelateerd aan apoptose, maar ook aan verhoogde IL-10-nivo's.

De onderzoekers vonden daarnaast significante hogere percentages granulocyten, lymfocyten en

High-throughput sequencing of plasma microRNA

in chronic fatigue syndrome/Myalgic Encephalomyelitis.

PLoS One. 2014 Sep 19;9(9):e102783. doi: 10.1371/journal.pone.0102783. eCollection 2014.

Brenu EW, Ashton KJ, Batovska J, Staines DR, Marshall-Gradisnik SM.

Abstract

BACKGROUND:

MicroRNAs (miRNAs) are known to regulate many biological processes and

their dysregulation has been associated with a variety of diseases

including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME).

The recent discovery of stable and reproducible miRNA in plasma

has raised the possibility that circulating miRNAs may serve as novel diagnostic markers.

The objective of this study was to determine the role of plasma miRNA in CFS/ME.

RESULTS:

Using Illumina high-throughput sequencing we identified

19 miRNAs that were differentially expressed in the plasma of CFS/ME patients

in comparison to non-fatigued controls.

Following RT-qPCR analysis,

we were able to confirm the significant up-regulation of

three miRNAs (hsa-miR-127-3p, hsa-miR-142-5p and hsa-miR-143-3p)

in the CFS/ME patients.

CONCLUSION:

Our study is the first to identify circulating miRNAs from CFS/ME patients and

also to confirm three differentially expressed circulating miRNAs in CFS/ME patients,

providing a basis for further study to find useful CFS/ME biomarkers.

PMID: 25238588

PMCID: PMC4169517

http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0102783&representation=PDF