Uit een posterpresentatie op de tienjaarlijkse retrovirus bijeenkomst (CRM) blijkt dat
de cytokines
die door XMRV-geinfekteerde afweercellen in vitro geproduceerd worden
goed stroken met het "cytokineprofiel" dat bij ME/CVS-patiënten gevonden wordt
(klik hier).
Dit lijkt te bevestigen dat XMRV bij (een deel van de) ME/CVS-patiënten een hoofdrol speelt.
Characterization of innate immune responses in Xenotropic Murine Leukemia Retrovirus-Related Virus (XMRV) infected individuals
J. Mikovits 1, V. Lombardi 1, K. Hagen 1, D. Goetz 1, M. Marshall 2, I. Barao-Silvestre 1
- Whittemore Peterson Institute, Research, Reno, USA
- University of Nevada, Microbiology and Immunology, Reno, USA
Centennial Retrovirus Meeting (CRM)
April 29 - May 4, 2010
Institute of Molecular Genetics, Prague, Czech Republic
Poster Presentations P62
Chronic fatigue syndrome (CFS)
is a debilitating disease
manifested by inflammatory sequelae
including innate immune activation, low natural killer cell numbers and function.
We recently demonstrated
the first direct isolation of
an infectious gammaretrovirus, XMRV,
from the blood of CFS patients.
We have developed
quantitative assays to detect XMRV replication and methods for infection in culture.
Moreover, we found evidence of
XMRV infection in >95% of the 141 CFS patients tested to date.
These data implicate
a role for XMRV infection in the pathogenesis of CFS.
We studied
the interaction of XMRV with the immune system,
first by identifying
cellular targets of XMRV ex vivo by flow cytometry (FCM).
We hypothesized
that NK cells could be a target of infection and
that infection of other PBMC cell types
could lead to dysfunction of NK cells
either directly or
indirectly through the upregulation of inflammatory cytokines and chemokines,
which are also a hallmark of this disease.
We
isolated leukocytes from XMRV infected individuals
analyzed phenotypic differences from uninfected controls by FCM.
Using purified
primary B, T monocyte macrophage and dendritic cells and
cell line models
we infected with XMRV and
profiled cytokine and chemokine expression by Luminex multiplex assays.
We analyzed
XMRV infection
by quantitative RT-PCR and intracellular FCM.
Infection of PBMC in vitro
results in
a cytokine signature similar to
that signature seen in the plasma of CFS patients.
These data advance our understanding of the pathogenesis of CFS and
may ultimately lead to new therapeutic interventions.
http://www.crm2010.org/images/stories/pdf/CRM2010-Programme-and-Abstract-book.pdf, blz. 57
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