Dr. Francis Ruscetti (van het Amerikaanse Kankerinstituut
NCI) presenteerde in april op een internationaal kongres
de resultaten van recent onderzoek naar XMRV bij ME/CVS-patienten.
Bij meer dan drie-kwart van de patiënten werd m.b.v. één of meer technieken XMRV vastgesteld.
Repeated Detection of Infectious Xenotropic Murine Virus-Related Virus (XMRV)
in Human Neoplasia and Neuroimmune Diseases
12th Intl. Conf. on Malignancies in AIDS and Other Acquired Immunodeficiences, Apr 26, 2010.
Francis Ruscetti, Vincent Lombardi, Max Pfost, Kathryn Hagen, Judy Mikovits.
http://www.prohealth.com/library/showarticle.cfm?libid=15496
(Note: This abstract, of a presentation given April 26 by National Cancer Institute researcher Frank Ruscetti, is listed in the conference
agenda & program book
as "Pathogenic Consequences of Xenotropic Murine Virus-Related Virus (XMRV) Expression in the Development of Chronic Diseases.
"Neoplasia" refers to abnormal tissue proliferation, as in a tumor.)
Background:
In 2006,
sequences of
a novel human retrovirus, XMRV,
were identified and
reported to be
associated with
a subset of
hereditary prostate cancer.
Although the public health implications
of this finding
were not immediately clear,
two recent papers show
XMRV is clearly a health concern.
- One clearly shows that XMRV expression in the proliferating prostate stroma and epithelium of prostate cancer patients [1].
- The second describes the detection of XMRV in about two-thirds of patients diagnosed with chronic fatigue syndrome [2].
We will present
data that
in these and
other neuroimmune diseases
and cancers,
the host mounts
a humoral response
[involving antibodies]
to XMRV and
infected patients
are viremic
[virus particles in the bloodstream].
Methods:
A combination of
classical retroviral methods,
including RT-PCR,
full-length genomic sequencing,
immunoblotting
of viral expression
in activated PBMC,
passage of infectious virus
in plasma and
PBMC
to indicator cell lines, and
presence of antibodies to XMRV
in plasma,
allowed XMRV detection
in more than 75% of
the CFS patients studied
Since then,
several publications in Europe
using DNA-PCR of
blood products
failed to detect
XMRV sequences in
patients with either disease and
have created considerable controversy.
Reliable methods
for
the biological and
molecular amplification
to detect XMRV
in
unstimulated blood cells and
plasma
have been developed.
Some DNA-PCR negative patient blood samples
represent false negatives, and
molecular analysis
using DNA
from unstimulated blood cells
is not yet sufficient
for XMRV identification.
Results:
In mice,
viruses
related to XMRV
cause B-cell lymphoma
usually by
insertional mutagenesis
activating
a cellular oncogene
as well as
causing chronic neurological diseases.
We will present
a case of
development of
such B cell lymphoma
in CFS patients.
XMRV-infected
individuals with
both neuroimmune disease and
cancer
develop an immune response to XMRV.
The isolation of
infectious XMRV
from prostate cancer patients
will be shown for the first time.
Pathogenic consequences of
this infection
will be discussed
Conclusion:
XMRV,
a retrovirus of
unknown pathogenic potential,
is infectious in humans.
References:
- Schlaberg et al.:
"XMRV is present in malignant prostate epithelium and is associated with prostate cancer."
Proc Natl Acad Sci U S A 106:16351, 2009.
- Lombardi et al.:
"Detection of an infectious retrovirus, XMRV, in blood cells of patients with chronic fatigue syndrome."
Science 326:585, 2009.
http://oham.cancer.gov/objects/pdf/2010ICMAOI_Program_Book.pdf
bladzijde 23
Met dank aan Erik.
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